Expressing:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Staining:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
RNA Sequencing:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Sequencing:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Migration:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Cell Culture:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Isolation:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Marker:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Western Blot:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Control:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
CRISPR:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Binding Assay:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Transfection:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Plasmid Preparation:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Molecular Weight:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
Immunostaining:Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization.
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature. ... The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY343 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes 14 Vol:. (1234567890) Scientific Reports | (2023) 13:17162 | https://doi.org/10.1038/s41598-023-44093-8 for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS..
Article Title: Immunoglobulin superfamily member 3 is required for the vagal neural crest cell migration and enteric neuronal network organization
Article Snippet: The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.The tissues were washed with PBS and treated with the blocking buffer (5% fetal bovine serum, 0.3% Triton-X-100 in PBS) for 1 h at room temperature.. The primary antibodies against NCAM1 (ab5032, 1:500, Merck), IGSF3 (AF4788, 1:750, R&D Systems),Tuj1 (AB18207, 1:1000, Abcam) and αSMA-CY3 (C6198, 1:300, Merck) were applied for 48 h at 4 °C followed by washes with 0.3% Triton-X-100 in PBS for the whole day and the secondary antibody donkey anti-rabbit Alexa Fluor 647 (A32795, 1:400, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (705–546-147, 1:400, Jackson ImmunoResearch) overnight at 4°C followed by washes for 3–4 h using 0.3% Triton-X-100 in PBS.. After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.After the secondary antibody incubation and washes, the tissues were post-fixed with 1% PFA for 10 min at RT followed by PBS wash.
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